Cyclooxygenase (COX) is a membrane-bound enzyme responsible for the oxidation of arachidonic acid to Prostaglandin G2 (PGG2) and the subsequent reduction of PGG2 to PHG2. These reactions are the first steps in the formation of a variety of prostanoids. COX has been shown to be expressed in at least two different isoforms, a constitutively expressed form, COX-1, and an inducible form, COX-2. COX-1 is thought to regulate a number of housekeeping functions, such as vascular hemostasis, renal blood flow, and maintenance of glomerular function. Inflammation mediators such as growth factors, cytokines, and endotoxins induce COX-2 expression in a number of cellular systems. This kit (Cat. # 27186) has improved stability and convenience compared to the former kit (Cat. # 17186). Its advantages are as follows: 1) The HRP-conjugated antibody has been reformulated from a lyophilized product to liquid (0.4 ml X 1). This change has improved the stability of the conjugate so that customers can be use it for 12 months even ifstored at 2–8 °C and can use it several times. 2) The assay range has changed from 2.15–275 ng/ml to 1.09–70 ng/mL (sensitivity is 0.25 ng/ml). Additionally, we recommend the customers to use IBLysis-I (Cat. # 19022) instead of TNE buffer for sample treatment. IBLysis-I was developed by improving TNE buffer solution for solubilization of cultured cells and tissue. It is ready to use for pretreatment of samples.
Trustpilot
2 days ago
2 weeks ago
