The fluorescent dyes, calcein acetoxymethylester (calcein AM) and DiIC12(3), can be used with Falcon* Corning FluoroBlok* cell culture inserts and insert systems to label cells when performing analyses such as tumor cell invasion, endothelial cell migration, and other cell-based assays. The calcein AM fluorescent dye can be used to fluorescently pre- and post-label viable cells to perform kinetic and endpoint experiments, respectively. The DiIC12(3) fluorescent dye can be used to fluorescently label viable cells for assays such as tumor cell invasion or endothelial cell migration. While cells labeled with calcein AM should be exposed to the dye for less than eight hours, DiIC12(3) can be used for several days in culture without adversely affecting cells.
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