Description
- Formula* per Liter: Potato Infusion .............................. 4.0g Dextrose .......................................20.0g Agar ............................................. 15.0g Final pH: 5.6 ± 0.2 at 25.0°C * Grams per liter may be adj. or formula supplemented to obtain desired performance Preparation: Mix 39.0 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes. If desired, the pH of the medium can be adjusted to 3.5 ± 0.1 prior to pouring plates by the addition of 14 mL of sterile 10% tartaric acid to melted sterile medium cooled to 45°-50.0°C. Avoid reheating medium after the addition of the tartaric acid solution. Quality Control Specifications: 1. The powder is homogeneous, free flowing and light beige. 2. Visually the prepared medium is clear to slightly hazy and pale to light yellow. 3. Expected cultural response after 5 days at 25-30.0°C. Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.
Use: Agar medium used for the isolation, identification and cultivation of fungi. Description: Potato Dextrose Agar is recommended for the cultivation and isolation of yeasts and molds from dairy and food products. Lowering the pH to 3.5 ± 0.1 inhibits bacterial growth and aids in the isolation of fungi.
